Gestational and hormonal regulation of human placental lipoprotein lipase

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Gestational and hormonal regulation of human placental lipoprotein lipase.

The fetal demand for FFA increases as gestation proceeds, and LPL represents one potential mechanism for increasing placental lipid transport. We examined LPL activity and protein expression in first trimester and term human placenta. The LPL activity was 3-fold higher in term (n = 7; P < 0.05) compared with first trimester (n = 6) placentas. The LPL expression appeared lower in microvillous me...

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Lipoprotein lipase: regulation and role in lipoprotein metabolism.

Humans and animals deficient in lipoprotein lipase (EC 3.1.1.34; LPL) develop a massive hypertriacylglycerolaemia (Brunzell, 1995). Hence, LPL-mediated hydrolysis of lipoprotein-triacylglycerols is a necessary step in lipoprotein metabolism. The reaction occurs at ‘binding-lipolysis’ sites at the vascular endothelium (Olivecrona & Olivecrona, 1995). Here the enzyme is anchored by interaction wi...

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Relationship between a lipoprotein lipase gene polymorphism in placental tissue and insulin resistance in patients with gestational diabetes mellitus.

The aim of this study was to investigate the relationship between a lipoprotein lipase (LPL) gene polymorphism in placental tissue and insulin resistance (IR) in patients with gestational diabetes mellitus. Using polymerase chain reaction-restriction enzyme fragment length polymorphism (PCR-RFLP) analysis, the LPL HindIII RFLP was examined in the placental tissue of 110 patients with gestationa...

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Functional characterization of a chimeric lipase genetically engineered from human lipoprotein lipase and human hepatic lipase.

Lipoprotein lipase (LPL) and hepatic lipase (HL) mediate the hydrolysis of triglycerides and phospholipids present in circulating lipoprotein particles and are essential for normal lipid metabolism. Both enzymes have a similar primary amino acid structure and share requirements for intact catalytic, lipid binding, and heparin binding domains. However, LPL and HL exhibit different substrate spec...

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Activation of lipoprotein lipase by lipoprotein fractions of human serum.

Triglycerides in fat emulsions are hydrolyzed by lipoprotein lipase only when they are "activated" by serum lipoproteins. The contribution of different lipoprotein fractions to hydrolysis of triglycerides in soybean oil emulsion was assessed by determining the quantity of lipoprotein fraction required to give half-maximal hydrolysis. Most of the activator property of whole serum from normolipid...

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 2006

ISSN: 0022-2275

DOI: 10.1194/jlr.m600098-jlr200